I GOT IT!

Hooray, and champers all round! I got two clones that are the right thing. All that is left is to send DNA to Adelaide for injection into flies, sequence said DNA and run around the lab screaming "WHOOP!" at the top of my lungs. No more cloning for me (it would seem) and lots of fly work and other things. I love molecular biology today. And in accordance with a request by VA (supplier of awesome chemically competent cells) I now renounce my love affair with electro competent cells and wholy embrace chemically competent cells as the way of the future. I shall use them forever more for my cloning endeavours and agree that they are much better and more efficient than their electro siblings.